Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Growth hormone secretagogue receptor (GHS-R) is expressed in type II but rarely in type III taste cells of circumvallate papillae. ( A – C ) PLCβ2-GFP, green; anti-GHS-R, red; and merged images, respectively. In ( C ), the yellow arrow points to a representative taste cell that expresses PLCβ2 but not GHS-R, and the white arrow highlights a PLCβ2-negative, GHS-R-positive taste cell. ( D – F ) GAD67-GFP, green; anti-GHS-R, red; and merged images, respectively. ( G ) Anti-GHS-R antibody incubated on a representative section of circumvallate papillae from a GHS-R-deficient mouse (negative control). Nuclear staining is shown in blue in all figures.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Incubation, Negative Control, Staining, Immunofluorescence

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: GHS-R is expressed in type II but rarely in type III taste cells of the fungiform papillae. ( A – C ) PLCβ2-GFP, green; anti-GHS-R, red; and merged images, respectively. ( D – F ) GAD67-GFP, green; anti-GHS-R, red; and merged images, respectively.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Immunofluorescence

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Body weight and food intake in wild-type (WT) and GHS-R-deficient mice on a high-fat diet. While not showing a significant difference in body weight ( A ), Ghsr −/− females consumed less compared to WT females ( B ). Alternatively, male Ghsr −/− mice ( C , D ) showed a decrease in body weight by week 5 of the HFD and no significant differences in food intake compared to their WT counterparts.* p -value < 0.05.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques:

Journal: Nutrients

Article Title: Ghrelin Receptors Enhance Fat Taste Responsiveness in Female Mice

doi: 10.3390/nu13041045

Figure Lengend Snippet: Linoleic acid responsiveness assessed in a conditioned taste aversion assay showed changes in Ghsr −/− female mice. ( A ). WT female mice (n = 15) revealed a significant aversion to LA at 10 µM, similar to that seen in WT males ( n = 15) ( B ). Ghsr −/− females ( n = 11) showed no significant differences in the LiCl compared to the NaCl group across all concentrations of LA ( C ). Male mice lacking GHS-R ( n = 18) showed aversion at 10 µM LA, similar to WT mice ( D ). WT and Ghsr −/− females ( E ) and males ( F ) exhibited similar lick ratios to the control solutions, sucrose (100 mM), and denatonium benzoate (3 mM). * p -value < 0.05, ** p -value ≤ 0.01, and *** p -value < 0.0001.

Article Snippet: After 3× 10 min rinses with PBS, the sections were blocked with 10% normal goat serum and 2% bovine serum albumin in PBST (PBS-0.05% Tween 20) for 1 h and incubated with 1:500 rabbit GHS-R (extracellular) (Alomone, Jerusalem, Israel) overnight in a blocking solution without Tween 20.

Techniques: Control